Skinner Laboratory

Michael K. Skinner, Ph.D.

BIOGRAPHICAL SKETCH

Skinner, Michael K.
Professor and Director


Education/Training

Warner Pacific College, Portland, OR              A.S.         1977       General Science
Reed College, Portland, OR                             B.A.         1979       Chemistry
Washington State University, Pullman, WA      Ph.D.       1982       Biochemistry
University of Toronto, Toronto, Ont., Canada   Post-doc  1982-84  Biochem/Physiol.


Research/Professional Experience:
1996-2008 Director, Center for Reproductive Biology, Washington State University, Pullman, Washington.
1996-2008 Professor, School of Molecular Biosciences, Washington State University, Pullman, Washington.
1991-1996 Member, the Reproductive Endocrinology Center and the Developmental Biology Program in Biological Sciences, University of California, San Francisco.
1991-1996 Associate Professor, Department of Obstetrics, Gynecology and Reproductive Sciences and Department of Physiology, University of California, San Francisco, CA.
1985-1991 Assistant Professor, Department of Pharmacology, Vanderbilt University, School of Medicine, Nashville, Tennessee. Research dealt with an analysis of cellular functions and cell-cell interactions in the testis and ovary.
1982-1984 MRC Postdoctoral Fellow, C.H. Best Institute, University of Toronto, Toronto, Ontario, Canada, in the laboratory of Dr. Irving B. Fritz. Research dealt with cell-cell interactions in the testis via basement membrane components and soluble peptides.
1979-1982 Research Assistant, Biochemistry/Biophysics Program, Washington State University, Pullman, Washington, in the laboratory of Dr. Michael D. Griswold. Research dealt with an investigation of Sertoli cell functions through a study of secreted proteins.
1978-1979 General Chemical Teaching Assistant and Research Assistant, Reed College, Portland, Oregon, in the laboratory of Dr. W. Block. Research consisted of studies on the enzyme kinetics of alcohol dehydrogenase as effected by irreversible inhibitors.


Publications: (selected from over 190)
Nilsson EE, Doraiswamy V, and MK Skinner (2003) Transforming Growth Factor-Beta (TGFβ1, TGFβ2 and TGFβ3)    expression during bovine ovarian antral follicle development. Molecular Reproduction and Development 66:237-246.
Kezele P and MK Skinner (2003) Regulation of ovarian primordial follicle assembly and development by estrogen and progesterone: endocrine model of follicle assembly. Endocrinology 144:3329-3337.
Cupp, A.S., Uzumcu, M, Phillips, B, Dirks, K, and MK Skinner (2003) Effect of Transient Embryonic In Vivo Exposure of the Endocrine Disruptor Methoxychlor on Embryonic and Postnatal Testis Development and Function. Journal of Andrology 24(5): 736-745.
Cupp AS, Uzumcu, M, and MK Skinner (2003) Chemotactic Role of Neurotropin 3 in the embryonic testis that facilitates morphological male sex determination. Biology of Reproduction 68:2033-2037.
Nilsson EE and MK Skinner (2003) Bone Morphogenetic Protein –4 acts as an ovarian follicle survival factor and promote primordial follicle development. Biology of Reproduction 69:1265-1272.
Chaudhary, J., Sadler-Riggleman, I and Skinner, M.K. (2004) Identification of a novel Sertoli cell gene product SERT that influences follicle stimulating hormone actions. Gene 324:79-88.
Nilsson EE and MK Skinner (2004) Kit ligand and basic fibroblast growth factor interactions in the induction of ovarian primordial to primary follicle transition. Molecular and Cellular Endocrinology 214:19-25.
Saxlund MA, Sadler-Riggleman I, and MK Skinner (2004) Role of Basic Helix-Loop-Helix (bHLH) and CREB Transcription Factors in the Regulation of Sertoli Cell Androgen Binding Protein Expression. Molecular Reproduction and Development 68:269-278.
Uzumcu M, Suzuki H, and MK Skinner (2004) Effect of the Anti-Androgenic Endocrine Disruptor Vinclozolin on Embryonic Testis Cord Formation and Postnatal Testis Development and Function. Reproductive Toxicology 18:765-774.
Chaudhary J, Sadler-Riggleman I and MK Skinner (2005) The Helix-Loop-Helix Inhibitor of Differentiation (Id) Proteins Induce Post-Mitotic Terminally Differentiated Sertoli Cells to Re-Enter the Cell Cycle and Proliferate. Biology of Reproduction 72:1205-1217.
Kezele PR, Ague J, Nilsson EE and MK Skinner (2005) Alterations in the ovarian transcriptome during primordial follicle assembly and development. Biology of Reproduction 72:241-255.
Cupp, A.S. and Skinner, M.K. (2005) Embryonic Sertoli Cell Differentiation. In: Sertoli Cell Biology eds.
M.D. Griswold and M.K. Skinner, Elsevier -Academic Press, San Diego, CA, pp. 43-70.
Skinner, M.K. (2005) Sertoli Cell Secreted Regulatory Factors. In: Sertoli Cell Biology eds. M.D. Griswold
and M.K. Skinner, Elsevier -Academic Press, San Diego, CA, pp. 107-120.
Chaudhary, J. and Skinner, M.K. (2005) Transcription Factors in Sertoli Cells. In: Sertoli Cell Biology eds.
M.D. Griswold and M.K. Skinner, Elsevier -Academic Press, San Diego, CA, pp. 251-280.
Skinner, M.K. (2005) Sertoli Cell-Somatic Cell Interactions. In: Sertoli Cell Biology eds. M.D. Griswold and M.K. Skinner, Elsevier -Academic Press, San Diego, CA, pp. 317-328.
Small CL, Shima JE, Uzumcu M,Skinner MK and MD Griswold (2005) Profiling gene expression during the differentiation and development of the murine embryonic gonad. Biology of Reproduction 72:492-501.
Ye X, Hama K, Contos J, Anliker B, Inoue A, Skinner MK, Suzuki H Amano T, Kennedy G, Arai H, Aoki J and Chun J. (2005) LPA3-mediated lysophosphatidic acid signaling in implantation and embryo spacing. Nature 435:104-108.
Anway M, Cupp AS, Uzumcu M and MK Skinner (2005) Epigenetic transgenerational actions of endocrine disruptors and male fertility. Science 308:1466-1469.
Meachem SJ, Ruwanpura SM, Ziolkowski J, Ague J, Skinner MK and KL Loveland. (2005) Developmentally distinct in vivo effects of FSH on proliferation and apoptosis during testis maturation. Journal of Endocrinology 186:429-446.
Muir T, Sadler-Riggleman I, Stevens JD, and MK Skinner (2005) Role of the basic helix-loop-helix protein ITF2 in the hormonal regulation of Sertoli Cell Differentiation. Molecular Reproduction and Development 73:491-500.
Muir T, Sadler-Riggleman I, Stevens JD, and MK Skinner (2005) Role of the basic helix-loop-helix transcription factor, scleraxis, in the regulation of Sertoli cell function and differentiation. Molecular Endocrinology.19:2164-2174.
Westfall S, and MK Skinner (2005) Inhibition Of Phosphatidylinositol 3-Kinase (PI3K) Sensitizes Ovarian Cancer Cells To Carboplatin And Allows Adjunct Chemotherapy Treatment. Molecular Cancer Therapeutics 4:1764-1771.
Kezele P, Nilsson EE and MK Skinner (2005) Keratinocyte growth factor acts as a mesenchymal factor to promote ovarian primordial to primary follicle transition. Biology of Reproduction 73:967-973.
Skinner MK. (2005) Regulation of primordial follicle assembly and development. Hum Reprod Update. 11:461.
Anway MD, Memon M, Uzumcu M, and MK Skinner (2006) Transgenerational effect of the endocrine disruptor vinclozolin on male spermatogenesis. Journal of Andrology 27:868-879.
Skinner MK and Anway MD (2006) Seminiferous cord formation and germ cell programming: epigenetic transgenerational actions of endocrine disruptors. Annals New York Academy of Science 1061:18-32.
Anway MD, Leathers C and MK Skinner (2006) Endocrine Disruptor Vinclozolin Induced Epigenetic Transgenerataional Adult Onset Disease. Endocrinology 147:5515-5523.
Chang HS, Anway MD, Rekow SS, and Skinner MK (2006) Transgenerational Epigenetic Imprinting of the Male Germ-Line by Endocrine Disruptor Exposure During Gonadal Sex Determination. Endocrinology 147:5524-5541.
Nilsson EE, Stanfield J, and MK Skinner (2006) Interactions between progesterone and tumor necrosis factoralpha in the regulation of primordial follicle assembly. Reproduction 132:877-886.
Nilsson EE, Detzel C, and MK Skinner (2006) Platelet-derived growth factor modulates the primordial to primary follicle transition in rat ovaries. Reproduction 131:1007-1015.
Skinner MK (2006) Epigenetic transgenerational actions of endocrine disruptors through the male germ-line. In: Male-Mediated Development Toxicology. Ed. D. Anderson and MH Brinkworth, Royal Society of Chemistry, Cambridge, UK, CH001, pp. 1-4.
Anway, MD and MK Skinner (2006) Epigenetic Transgenerational Actions of Endocrine Disruptors. Endocrinology 147:S43-S49.
Nilsson EE, Rogers N and MK Skinner (2007) Actions of the anti-müllerian hormone (AMH) on the ovarian transcriptome to inhibit primordial to primary follicle transition. Reproduction 134:209-221.
Jirtle RL and MK Skinner (2007) Environmental Epigenomics and Disease Susceptibility. Nature Genetics Rev. 8:253-262.
Crews D, Gore AC, Hsu TS, Dangleben NL, Spinetta M, Schallert T, Anway MD and Skinner MK (2007) Treansgenerational epigenetic imprints on mate preference. Proc Natl Acad Sci 104:5942-5946.
Clement TM, Anway MD, Uzumcu M and MK Skinner. (2007) Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: candidate regulatory genes. Reproduction 134:455-472.
Skinner MK (2007) Epigenetic Transgenerational Toxicology and Germ Cell Disease. International Journal of Andrology 30:393-397.
Skinner MK (2007) Epigenetic Transgenerational Disease Etiology. Pediatric Research 61:48R-50R.
Anway MD and MK Skinner (2007) Epigenetic Transgenerational Actions of Vinclozolin on the Development of Disease and Cancer. Critical Reviews in Oncogenesis 13:75-82.
Anway MD and MK Skinner (2008) Epigenetic Programming of the Germ Line: Effects of Endocrine Disruptors on the Development of Transgenerational Disease. Reproductive Biomedicine Online. 16:23-25.
Westfall S, Nilsson EE and MK Skinner (2008) Role of triptolide as an adjunct chemotherapy for ovarian cancer. Chemotherapy 54:67-76.
Anway MD, Rekow SS, and MK Skinner (2008) Transgenerational epigenetic programming of the testis transcriptome by endocrine disruptor exposure at sex determination. Genomics 91:30-40.
Skinner MK (2008) What is an epigenetic transgenerational phenotype? F3. Reproductive Toxicology. 25:2-6.
Anway MD and Skinner MK (2008) Epigenetic transgenerational effects of the endocrine disruptor vinclozolin on the prostate transcriptome and adult onset prostate disease. Prostate. 68:517-529.
Muir T, Wilson-Rawls J, Stevens JD, Rawls A, Schweitzer R, Kang C, Skinner MK. (2008) Integration of CREB and bHLH transcriptional signaling pathways through direct heterodimerization of the proteins: Role in muscle and testis development. Mol Reprod Dev. 75(11):1637-52.
Skinner MK, Schmidt M, Savenkova MI, Sadler-Riggleman I, Nilsson EE. (2008) Regulation of granulosa and theca cell transcriptomes during ovarian antral follicle development. Mol Reprod Dev. 75(9):1457-72.
Stevens JD, Roalson EH, and Skinner MK. (2008) Phylogenetic and expression analysis of the basic helix-loop-helix transcription factor gene family: genomic approach to cellular differentiation. Differentiation. 2008 Jun 13. [Epub ahead of print]
Dole G, Nilsson EE, Skinner MK. (2008) Glial-derived neurotrophic factor promotes ovarian primordial follicle development and cell-cell interactions during folliculogenesis. Reproduction. 135(5):671-82.
Nilsson EE, Anway MD, Stanfield J, Skinner MK. (2008) Transgenerational epigenetic effects of the endocrine disruptor vinclozolin on pregnancies and female adult onset disease. Reproduction. 135(5):713-21.
Ye X, Skinner MK, Kennedy G, Chun J. (2008) Age-dependent loss of sperm production in mice via impaired lysophosphatidic acid signaling. Biol Reprod. 79(2):328-36.
Memon MA, Anway MD, Covert TR, Uzumcu M, Skinner MK. (2008) Transforming growth factor beta (TGFbeta1, TGFbeta2 and TGFbeta3) null-mutant phenotypes in embryonic gonadal development. Mol Cell Endocrinol. 2008 Aug 26. [Epub ahead of print]
Anway MD, Rekow SS, Skinner MK. (2008) Comparative anti-androgenic actions of vinclozolin and flutamide on transgenerational adult onset disease and spermatogenesis. Reprod Toxicol. 2008 Aug 13. [Epub ahead of print]
Hahn KL, Beres B, Rowton MJ, Skinner MK, Chang Y, Rawls A, Wilson-Rawls J. (2008) A deficiency of lunatic fringe is associated with defects of the rete testis. Reproduction. 2008 [Epub ahead of print]


Research Projects:


ACTIVE
• NIH R01 HD 43841-01, 4/1/03-3/30/08, 25%, Cell-Cell Interactions and Testis Development $ 1,321,200
This grant deals with the growth factors involved in the onset and progression of embryonic sex
determination and testis development. No overlap exists between this grant and the current proposal.
• NIH R01 HD 043093, 4/1/06-3/30/11, 15%, Ovarian Primordial Follicle Development $1,450,000 This
project deals with the cellular and molecular control of ovarian primordial follicle development. No
overlap exists between this grant and the current proposal.
• NIH R01 ES012974-01, 12/1/04-11/31/08, 25%, Transgenerational Epigenetic Endocrine Disruptor
Actions on Male Fertility. $1,284.500. This project deals with the examination of endocrine disruptors on
testis development and male fertility. This grant investigates the mechanisms involved in the phenomena
and the current proposal is a competitive renewal of this grant.
• DOD, USAMRMC BAA Log No 07087010, 25%, Epigenetic Origins of Disease and Impact of
Environmental Toxicants in Iraq Theater of Operations
This project investigates the actions of four different mixtures of military relevant compounds to examine
potential transgenerational actions on adult onset disease. This proposal examines different compounds
and not mechanism, so no overlap exists.


PENDING
• NIH R01 HD 43841-06, 25%, Cell-Cell Interactions and Testis Development
This project investigates transcription factor and growth factor mediated cell-cell interactions involved in
the onset and progression of embryonic gonadal sex determination and testis development.
• NIH R01 ES012974-01, 12/1/08-11/31/13, 25%, Transgenerational Epigenetic Endocrine Disruptor
Actions on Male Fertility. This project deals with the examination of endocrine disruptors on testis
development and male fertility. This grant investigates the mechanisms involved in the phenomena and
the current proposal is a competitive renewal of this grant.

Skinner Laboratory, PO Box 644234, Washington State University, Pullman WA 99164-4234, 509-335-1524, Contact Us