Skinner Laboratory

Ramji Bhandari, Ph.D

Position: Postdoctoral Research Fellow

http://www.ramjibhandari.net


Education and Training
Bangladesh Agricultural University, Bangladesh    B.Sc.(Hons.) 1997              Fish Biology
Bangladesh Agricultural University, Bangladesh    M.S.              1998              Fish Biology
Hokkaido University, Sapporo, Japan                     Ph.D.             2002             Comparative Endocrinology


Work Experience:
1997-1998 Teaching Assistant with Dr. Arshad Hossain, Faculty of Fisheries, Bangladesh
                  Agricultural University, Bangladesh
1998-1999 Research Assistant with Dr. Hiroshi Ueda, Hokkaido University, Sapporo, Japan
1999-2002 Ph.D. Fellow with Dr. Kohei Yamauchi, Hokkaido University, Hakodate, Japan
2002-2003 Tentative Lecturer with Dr. Masaru Nakamura, University of the Ryukyus,
                  Okinawa, Japan
2003-2005 JSPS Postdoctoral Fellow with Dr. Yoshitaka Nagahama, National Institute for
                  Basic Biology, Japan
2005-2006 Postdoctoral Research Associate with Dr. Yoshitaka Nagahama, National Institute
                  for Basic Biology, Japan
2006- to date Postdoctoral Research Associate with Dr. Michael Skinner, Washington State University, Pullman, WA.


REVIEWS
Nakamura M. Alam, M.A., Kobayashi, Y. and Bhandari, R.K. (2007). The role of sex hormones in sex
change of grouper, Epinephelus merra. Journal of Marine Science and Technology-Taiwan, Special
issue 15: 23-27.


Nakamura M., Kobayashi, Y., Miura, S. Alam, M.A., and Bhandari R.K. (2006). Sex change in coral
reef fish. Fish Physiology and Biochemistry 31: 117-122.


Bhandari, R.K., Alam, MA., Soyano, K., Nakamura, M. (2007). Female-male sex change in grouper. in
preparation.


ARTICLES IN PREPARATION
Bhandari, R.K., and et. al. (2008). Generation of medaka transgenic lines with GFP fluorescence in
the ovarian follicle cells: a tool for studying the development of ovarian follicle cells and female sex
differentiation (in preparation).


Bhandari, R.K., and et. al. (2008). Neural and peripheral responses of brain aromatase to
environmental stressors in the living medaka. (in preparation).


Bhandari, R.K., and et. al. (2008). Gonochoristic fish maintain their sexual plasticity to adulthood.
*Equal contribution (in preparation)


Bhandari, R.K., and et. al. (2008). Methyl-testosterone treatment suppresses reproductive axis of the
pre-pubertal Cichlid fish, Nile tilapia. (in preparation)


PEER-REVIEWED ARTICLES
Kojima, Y. Bhandari, R.K., Kobayashi, Y. and Nakamura M. (2008). Male-to-female sex reversal of
primary male wrasse with estrogen treatment. General and Comparative Endocrinology. 156:628-632.


Westring, C.G., Ando, H., Kitahashi, T., Bhandari, R.K., Ueda, H., Urano, A., Dores, R.M., Sher,
A.A. and Danielson, P.B. (2008). Seasonal changes in CRF-I and urotensin I transcript levels in masu
salmon: Correlation with cortisol secretion during spawning. General and Comparative Endocrinology,
155: 126-140.


Komatsu, T. Bhandari, R.K., Nakamura, M. (2006). Acceleration of precocious spermatogenesis in
under-yearling Golden Rabbitfish (Siganus guttatus, Bloch) by GnRHa treatments. Aquaculture 257:
558-565.


Alam, M.A., Bhandari, R.K. ,Kobayashi, Y., Soyano K., and Nakamura, M., (2006). Induction of sex
change within two full moons during breeding season and spawning in grouper. Aquaculture 255: 532-
535.


Alam, M.A., Bhandari, R.K., Kobayashi, Y., Soyano, K., and Nakamura, M. (2006). Changes in
androgen-producing cell size and circulating 11-ketotestosterone levels during female-to-male sex
change in Honeycomb grouper (Epinephelus merra). Molecular Reproduction and Development.
73(2):206-14.


Bhandari, R.K., Alam, M.A.,, Soyano K., and Nakamura, M., (2006). Induction of female-to-male sex
change in the honeycomb grouper (Epinephelus merra) by 11-ketotestosterone treatments. Zoological
Science 23: 65-69.


Bhandari, R.K., Nakamura, M., Kobayashi, T., and Nagahama, Y. (2006). Suppression of
steroidogenic enzyme expression during androgen-induced sex reversal in Nile tilapia (Oreochromis
niloticus). General and Comparative Endocrinology 145: 20-24.


Bhandari, R.K., Alam, M.A., Higa, M. Soyano, K., Nakamura, M. (2005). Evidence that estrogen
regulates the sex change of honeycomb grouper (Epinephelus merra), a protogynous hermaphrodite
fish. Journal of Experimental Zoology 330A: 497-503.


Jodo, A.., Kitahashi, T., Taniyama, S., Bhandari, R.K., Ueda, H., Urano, A. and Ando, H. (2005).
Seasonal variations in expression of five subtypes of gonadotropin-releasing hormone receptor genes
in the brain of masu salmon from immaturity to spawning. Zoological Science 22: 1331-1338.


Luo, Q., Ban, M., Ando, H., Kitahashi, T., Bhandari, R.K., McCormick, S.D. and Urano, A. (2005).
Distinct effects of 4-nonylphenol and estrogen-17beta on expression of estrogen receptor a gene in
smolting sockeye salmon. Comparative Biochemistry and Physiology. 140C: 123-130.


Alam, M.A., Komuro, H., Bhandari, R.K. , Nakamura, S., Soyano, K., and Nakamura, M. (2005).
Immunohistochemical evidence identifying the site of androgen production in the ovary of the
protogynous grouper Epinephelus merra . Cell and Tissue Research 320: 323-329.


Bhandari, R.K., Komuro, H., Higa, M., and Nakamura, M. (2004). Sex inversion of sexually immature
honeycomb grouper (Epinephelus merra) by aromatase inhibitor. Zoological Science 21 (3): 305-310.


Bhandari, R.K., Higa, M., Nakamura, S. and Nakamura, M. (2004). Aromatase inhibitor induces
complete sex change in a protogynous honeycomb grouper, Epinephelus merra. Molecular
Reproduction and development, 67(3): 303-307.


Miura, S., Komatsu, T., Higa, M., Bhandari, R.K., Nakamura, S. and Nakamura, M. (2003). Gonadal
sex differentiation in protandrous anemone fish, Amphiprion clarkii. Fish Physiology and Biochemistry
28: 165-166.


Nakamura, M., Bhandari, R.K. and Higa, M. (2003). The role estrogens play in sex differentiation and
sex changes of fish. Fish Physiology and Biochemistry 28: 113-117 (Review).


Bhandari, R.K. Komuro, H., Nakamura, S., Higa, M., and Nakamura (2003). Gonadal restructuring
and correlative steroid hormone profiles during natural sex change in protogynous honeycomb
grouper, Epinephelus merra. Zoological Science 20: 1399-1404.


Bhandari, R.K., Higa, M., Komuro, H., Nakamura, S. and Nakamura, M. (2003). Treatments with an
aromatase inhibitor induces complete sex change in protogynous honeycomb grouper (Epinephelus
merra). Fish Physiology and Biochemistry 28: 141-142.


Bhandari, R.K., Taniyama, S., Kitahashi, T., Ando, H., Yamauchi, K., Zohar, Y., Ueda, H., and Urano,
A. (2003). Seasonal changes of responses to gonadotropin-releasing hormone analog in expression of
growth hormone/prolactin/somatolactin genes in the pituitary of masu salmon. General and
Comparative Endocrinology 130: 55-63.


Bhandari, R.K., Ushikoshi, I., Fukuoka, H., Koide, N., Yamauchi, K., and Ueda, H. (2002). Effects of
Rhizopus extract administration on somatic growth and sexual maturation in lacustrine sockeye
salmon, Oncorhynchus nerka. Fisheries Science. 68 (4): 776-782.


INVITED SEMINARS

July 05, 2006. Past, present and future of our research on sexual plasticity in fish. Invited Seminar at
Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, Japan.
(Host: Prof. Masaru Nakamura, UoR).


March 01, 2006. Sexual plasticity in fish. Department of Biological Sciences, University of Idaho,
Moscow, USA. (Host: Department of Biological Sciences, UI, Moscow ID, USA).


February 27, 2006. Sexual plasticity in fish. Northwest Fisheries Science Center, NOAA-National
Marine Fisheries Service, Seattle WA, USA. (Host: Dr. Penny Swanson, NOAA).


January 27, 2006. Sexual plasticity in fish. Special lecture to graduate students and postdocs at
University of the Ryukyus, Okinawa, Japan. (Host: Prof. Masaru Nakamura, UoR)


June 21, 2005. Consequences of estrogen removal in female fish. Invited Seminar at Sesoko Station,
Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, Japan. (Host: Prof. Masaru
Nakamura, UoR).


November 8, 2005. A primary factor regulating female to male sex change in honeycomb grouper
(Epinephelus merra). Joint Symposium on fish reproduction and aquaculture. International Coral Reef
Research and Monitoring Renter, Ishigaki, Japan. (Host: Prof. Masaru Nakamura, UoR)

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